ARPE-19 Human Retinal Pigment Epithelial Cells

The cell line was derived from the retina of a 19-year-old healthy man who was killed in a car accident and was established by Amy Aotaki-Keen in 1986. This cell line expresses molecular markers specific to retinal pigment cells, such as intracellular retinol binding protein and PRE-65. It was identified as free of mycoplasma/STR and put into storage.

Tissues and Cell Type eyes; Retinal pigment epithelium; retina

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM high glucose (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0118)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=8698076; DOI=10.1006/exer.1996.0020

Dunn K.C., Aotaki-Keen A.E., Putkey F.R., Hjelmeland L.M.

ARPE-19, a human retinal pigment epithelial cell line with differentiated properties.

Exp. Eye Res. 62:155-169(1996)

PubMed=11504951; DOI=10.1073/pnas.171266298

Lund R.D., Adamson P., Sauve Y., Keegan D.J., Girman S.V., Wang S., Winton H.L., Kanuga N., Kwan A.S.L., Beauchene L., Zerbib A., Hetherington L., Couraud P.-O., Coffey P., Greenwood J.

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.

Proc. Natl. Acad. Sci. U.S.A. 98:9942-9947(2001)

PubMed=14551534

Rogojina A.T., Orr W.E., Song B.K., Geisert E.E. Jr.

Comparing the use of Affymetrix to spotted oligonucleotide microarrays using two retinal pigment epithelium cell lines.

Mol. Vis. 9:482-496(2003)

PubMed=16262907; DOI=10.1186/1471-2415-5-25

Sharma R.K., Orr W.E., Schmitt A.D., Johnson D.A.

A functional profile of gene expression in ARPE-19 cells.

BMC Ophthalmol. 5:25-25(2005)

PubMed=21697133; DOI=10.1167/iovs.11-7479

Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.

Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011)

DOI=10.5897/IJBMBR2013.0154

Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.

Cell growth curves for different cell lines and their relationship with biological activities.

Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=24251032; DOI=10.1155/2013/216359

Pasovic L., Utheim T.P., Maria R., Lyberg T., Messelt E.B., Aabel P., Chen D.F., Chen X., Eidet J.R.

Optimization of storage temperature for cultured ARPE-19 cells.

J. Ophthalmol. 2013:216359-216359(2013)

PubMed=25177495; DOI=10.1155/2014/801787

Kuznetsova A.V., Kurinov A.M., Aleksandrova M.A.

Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium.

J. Ophthalmol. 2014:801787-801787(2014)

PubMed=27499609

Wang Y., Sang A.-M., Zhu M.-H., Zhang G.-W., Guan H.-J., Ji M., Chen H.

Tissue factor induces VEGF expression via activation of the Wnt/beta-catenin signaling pathway in ARPE-19 cells.

Mol. Vis. 22:886-897(2016)

PubMed=28978645; DOI=10.1074/jbc.M117.812677

Chiang C.-K., Tworak A., Kevany B.M., Xu B., Mayne J., Ning Z., Figeys D., Palczewski K.

Quantitative phosphoproteomics reveals involvement of multiple signaling pathways in early phagocytosis by the retinal pigmented epithelium.

J. Biol. Chem. 292:19826-19839(2017)

PubMed=29476476; DOI=10.1007/978-1-4939-7680-5_17

Fasler-Kan E., Aliu N., Wunderlich K., Ketterer S., Ruggiero S., Berger S., Meyer P.

The retinal pigment epithelial cell line (ARPE-19) displays mosaic structural chromosomal aberrations.

Methods Mol. Biol. 1745:305-314(2018)

PubMed=31254428; DOI=10.1111/ceo.13578

Churm R., Dunseath G.J., Prior S.L., Thomas R.L., Banerjee S., Owens D.R.

Development and characterization of an in vitro system of the human retina using cultured cell lines.

Clin. Exp. Ophthalmol. 47:1055-1062(2019)

PubMed=31412689; DOI=10.3390/pharmaceutics11080412

Hellinen L., Pirskanen L., Tengvall-Unadike U., Urtti A., Reinisalo M.

Retinal pigment epithelial cell line with fast differentiation and improved barrier properties.

Pharmaceutics 11:412.1-412.12(2019)