HFL1 Human Embryonic Lung Fibroblasts

This cell is a stable diploid cell. The sample age is 16 weeks fetus, male. It was identified as free of mycoplasma/STR and put into storage.

Tissue and Cell Type: lung

Morphology: Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

F12K (MD0400)+10% fetal bovine serum (SD0200)+1% GA (SD0038)

Link to complete medium: F12K complete medium (MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7372635

Breul S.D., Bradley K.H., Hance A.J., Schafer M.P., Berg R.A., Crystal R.G.

Control of collagen production by human diploid lung fibroblasts.

J. Biol. Chem. 255:5250-5260(1980)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222

Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.

Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.

Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=22481983; DOI=10.2174/1874091X01206010016

Chauhan V., Howland M., Wilkins R.

Effects of alpha-particle radiation on microRNA responses in human cell-lines.

Open Biochem. J. 6:16-22(2012)