SV40 MES 13 Mouse Glomerular Mesangial Cells

The cytoskeletal staining of this cell line highlights actin, and there are abundant parallel microfilaments in the cytoplasm. It is reported that they will contract in the presence of 1uM angiotensin II. The cells were cloned in soft agar, SV40 big T antigen was positive, and were identified and stored without mycoplasma.

Tissue and Cell Type: glomerular mesangial cells

Morphology: cardiac fibroblasts like, adherent growth

Medium and Culture Conditions

90% DMEM/F12 (3:1) medium+5% fetal bovine serum (SD0200)+14mM HEPES (SD0009)+1% GA (SD0038)

Link to complete medium: DMEM/F12 complete medium (MD0607)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=2835539; DOI=10.1038/ki.1988.53

MacKay K., Striker L.J., Elliot S., Pinkert C.A., Brinster R.L., Striker G.E.

Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice.

Kidney Int. 33:677-684(1988)

PubMed=17178593; DOI=10.1080/13813450601093518

Cheng D.W., Jiang Y., Shalev A., Kowluru R., Crook E.D., Singh L.P.

An analysis of high glucose and glucosamine-induced gene expression and oxidative stress in renal mesangial cells.

Arch. Physiol. Biochem. 112:189-218(2006)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)