BEnd. 3 Mouse Brain Microvascular Endothelial Cells

The cells were transformed by NTKmT retrovirus expressing the T antigen of polyoma virus. The expression of von Willebrand factor and the uptake of fluorescent-labeled low density lipoprotein (LDL) were observed to confirm its endothelial cell characteristics. BEnd. 3 cells can induce the expression of Peyer's node endothelial cell receptor, mucosal vascular settlement factor (MAdCAM-1) and endothelial cell selectin in lymphocytes by cytokines and lipopolysaccharide (LPS). The induction of tumor necrosis factor a (TNF alpha), interleukin 1 (IL-1) or LPS is concentration and time dependent. The bEnd. 3 cells with earlier generations expressed MAdCAM-1 on the surface when they were not stimulated, but they did not express it after 30 generations. Cell adhesion molecule-1 (ICAM-1) was continuously expressed and increased after LPS, IL-1 and TNF a treatment. Vascular cell adhesion molecule-1 (VCAM-1) was continuously expressed before 30 generations, but not after 30 generations. Tumor necrosis factor a (TNF alpha) in bEnd. 3 cells can induce the expression of P-selectin, which is stronger after 30 generations.

Organization source/Issue and Cell Type：Brain; Cerebral cortex; Disease: Endothelioma

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

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