EL4 Mouse Lymphoma Cells

EL4 was established from lymphoma induced by 9,10-dimethyl-1,2-benzanthracene in C57BL mice. It can resist 0.1 mM hydrocortisone and is sensitive to 20 mcg/ml PHA. Another sub-strain (EL4.IL-2, ATCC TIB-181) can produce high level of IL-2. The detection showed that the limb bone malformation virus (mouse pox) was negative. Through mycoplasma detection in this library.

Animal species/Organism mice

Tissue and Cell Type T lymphocytes; lymphoma

Morphology lymphoblasts, suspended growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

85%DMEM(MD0100)+10%FBS(SD0200)+1%GA(SD0038)

Complete medium: DMEM high sugar complete medium (MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

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Gorer P.A.

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Mohit B., Fan K.

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PubMed=30084848; DOI=10.1038/sdata.2018.157

Schuster H., Shao W., Weiss T., Pedrioli P.G.A., Roth P., Weller M., Campbell D.S., Deutsch E.W., Moritz R.L., Planz O., Rammensee H.-G., Aebersold R., Caron E.

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Sci. Data 5:180157-180157(2018)