YAC-1 Mouse Lymphoma Cells

YAC-1 is a lymphoma. Moloney leukemia virus (MLV) is inoculated into newborn A/Sn mice. Cells are sensitive to the activity of NK cells and are very useful for the detection of NK cell activity. The detection showed that the limb bone malformation virus (mouse pox) was negative. Through mycoplasma detection in this library.

Animal species/Organism mice

Tissue and Cell Type Moloney murine leukemia virus (Mo-MuLV) infection; lymphoma

Morphology lymphoblasts, suspended growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=1234049; DOI=10.1002/eji.1830050208

Kiessling R., Klein E., Wigzell H.

'Natural' killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype.

Eur. J. Immunol. 5:112-117(1975)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)