RAW 264.7 Mouse Monocyte Macrophage Leukemia Cells

The cell line was constructed from tumor tissue induced by Abelson mouse leukemia virus, and is one of the most commonly used inflammatory cell models in scientific research. This cell is easy to proliferate, highly efficient in DNA transfection, and sensitive to RNA interference. It is often used to transfect host cells and replicate murine norovirus. The sIg -, Ia - and Thy-1.2 antigens on the cell surface were negative. It is reported that after the establishment of the cell line, it has no secretion and no virus particles can be detected, and the XC spot formation test is negative. It can decompose sheep red blood cells and tumor target cells in an antibody-dependent manner. LPS or PPD treatment can induce the decomposition of red blood cells after 2 days, but has no effect on tumor target cells. The test showed that the mouse pox virus was negative. Each batch passed the detection of mycoplasma in this library, and the result was negative. The results of STR identification of the cell were as follows: ① The STR typing results of the cell DNA of the strain showed that the amplified map was clear and the typing results were good: 18-3:18; 9-2：14.3； 6-7：12,13； 5-5：14,15； X-1：23.3； 15-3：22.3； 12-1：15.3； 6-4：16.3，17.3； CSF1PO: none; VWA: none; 4-2：20.3； Jarid1：X，Y。 ② The cell line is indeed a mouse cell without human cell contamination

Animal species/Organism mice

Gender Male

Tissue and Cell Type murine leukemia virus induced tumor; ascites

Morphology: monocyte/macrophage like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0118)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=34952228; DOI=10.1016/j.bone.2021.116301

RANKL inhibition halts lesion progression and promotes bone remineralization in mice with fibrous dysplasia(2021）

Cellular uptake, transport mechanism and anti-inflammatory effect of cyanidin-3-glucoside nanoliposomes in Caco-2/RAW 264.7 co-culture model，https://doi.org/10.3389/fnut.2022.995391