Sertoli Cells Of TM4 Mouse Testes

Product Description:

    Under the action of FSH, the production of cAMP increased, but there was no response to LH; Compared with the primary Sertoli cells, the responsiveness of the cells to FSH decreased. It is reported that the production of constitutive plasminogen activator of this cell is very low, but it can be stimulated by FSH, and can be significantly increased by retinoic acid stimulation. The cells can produce retinol-binding protein, tissue plasminogen activator and transferrin; It expresses FSH receptor, androgen receptor and progesterone receptor. Mouse pox virus is negative.

Animal species/Organism mice

Tissue and Cell Type testis

Morphology epithelial cell-like; Adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM high glucose (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM high sugar complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=6774781; DOI=10.1095/biolreprod23.1.243

Mather J.P.

Establishment and characterization of two distinct mouse testicular epithelial cell lines.

Biol. Reprod. 23:243-252(1980)

PubMed=15541572; DOI=10.1016/j.mce.2003.05.001

Rahman N.A., Huhtaniemi I.T.

Testicular cell lines.

Mol. Cell. Endocrinol. 228:53-65(2004)

PubMed=26900862; DOI=10.1159/000444157

Schmid M., Guttenbach M., Steinlein C., Wanner G., Houben A.

Cytogenetic characterization of the TM4 mouse Sertoli cell line. II chromosome microdissection, FISH, scanning electron microscopy, and confocal laser scanning microscopy.

Cytogenet. Genome Res. 147:135-143(2015)