HFF-1 Skin Fibroblasts

It is taken from the foreskin fibroblasts of a newborn boy from a foreigner, and the number of cells per branch is 0.4 × 106, which can be used by feeder cells of hES such as HN4, HN1, H1, H9, etc. P18 generation when frozen. It is recommended to use before P30 generation. Each batch passed the detection of mycoplasma in this library, and the result was negative. The STR test of this library is correct. STR identification result: D5S818: 11,11; D13S317: 11,13； D7S820: 9,10,12； D16S539: 10,12； vWA: 14,15,17,19； THO1: 7,9,9.3； Amelogenin: X,Y； TPOX: 8,11； CSF1PO: 7,11,12,13。

Animal species/Organism Human

Gender Newborn Boys

Tissue and Cell Type: obtained from the foreskin of a newborn boy from a foreigner

Morphology: Fibroblast-like; Adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

85% DMEM (MD0100)+15% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0119)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=18415935; DOI=10.1387/ijdb.082590le

Eiselleova L., Peterkova I., Neradil J., Slaninova I., Hampl A., Dvorak P.

Comparative study of mouse and human feeder cells for human embryonic stem cells.

Int. J. Dev. Biol. 52:353-363(2008)