OKT 3 Hybridoma (anti-CD3)

Mice were immunized with human peripheral blood lymphocytes. The spleen cells were taken and fused with P3x63Ag8.U1 myeloma cells. It was put into storage after identification without mycoplasma.

Animal species/Organism Human/Mouse

Tissues and Cell Type: hybridoma; B lymphocyte

Morphology lymphoblasts, suspended growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

80% IMDM (MD0700)+20% fetal bovine serum (SD0200)+G/A (SD0038)

IMDM complete medium (article number: MD0703)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=314668; DOI=10.1126/science.314668

Kung P.C., Goldstein G., Reinherz E.L., Schlossman S.F.

Monoclonal antibodies defining distinctive human T cell surface antigens.

Science 206:347-349(1979)

Patent=US4361549

Kung P.C., Goldstein G.

Complement-fixing monoclonal antibody to human T cells, and methods of preparing same.

Patent number US4361549, 30-Nov-1982