OK Opossum Kidney Cell

This cell line was originally designed to provide X chromosome for the study of X chromosome inactivation, and then it was found that it was an excellent cell culture model for renal proximal tubular epithelial cells. This cell expresses a variety of receptors in culture and is widely used as a model for studying these receptors. It was put into storage after identification without mycoplasma.

Animal species/Organism opossum

Tissue and Cell Type renal proximal convoluted tubules, epithelial cells

Morphology epithelial cell-like; Adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=566717; DOI=10.1007/BF02616032

Koyama H., Goodpasture C., Miller M.M., Teplitz R.L., Riggs A.D.

Establishment and characterization of a cell line from the American opossum (Didelphys virginiana).

In Vitro 14:239-246(1978)

CLPUB00465

Esposito D.

Cytogenetic and mutagenic studies with an opossum cell line (Didelphys virginiana).

Thesis PhD (1982), Fordham University, United States

PubMed=6394469; DOI=10.1007/BF02619628

Keith D.H., Teplitz R.L., Riggs A.D.

Metaphase synchronization and chromosome preparation from the OK opossum cell line having a potentially isolatable X chromosome.

In Vitro 20:833-836(1984)

PubMed=1802405; DOI=10.1016/0309-1651(91)90094-Y

Courjault F., Gerin B., Leroy D., Chevalier J., Toutain H.

Morphological and biochemical characterization of the opossum kidney cell line and primary cultures of rabbit proximal tubule cells in serum-free defined medium.

Cell Biol. Int. Rep. 15:1225-1234(1991)

PubMed=28615248; DOI=10.1152/ajprenal.00228.2017

Eshbach M.L., Sethi R., Avula R., Lamb J., Hollingshead D.J., Finegold D.N., Locker J., Chandran U.R., Weisz O.A.

The transcriptome of the Didelphis virginiana opossum kidney OK proximal tubule cell line.

Am. J. Physiol. Renal Physiol. 313:F585-F595(2017)

PubMed=30379374; DOI=10.1113/EP087217

Guillen N., Caldas Y.A., Levi M., Sorribas V.

Identification and expression analysis of type II and type III Pi transporters in the opossum kidney cell line.

Exp. Physiol. 104:149-161(2019)