HARA-B Human Lung Squamous Cell Carcinoma Cells

Initially human cells, but the cell line was established from the metastatic organ (bone) of nude mice. The cell line highly expressed PTHrP, and intracardiac injection of nude mice can widely induce bone metastasis. Subcutaneous transplantation of cells in mice can cause hypercalcemia.

Animal species/Organism Human

Tissue and Cell Type Lung, Squamous Cell Carcinoma

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Statement: The product is for scientific research only

PubMed=8752176

Iguchi H., Tanaka S., Ozawa Y., Kashiwakuma T., Kimura T., Hiraga T., Ozawa H., Kono A.

An experimental model of bone metastasis by human lung cancer cells: the role of parathyroid hormone-related protein in bone metastasis.

Cancer Res. 56:4040-4043(1996)

PubMed=11668474; DOI=10.1002/ijc.1425

Iguchi H., Onuma E., Sato K., Sato K., Ogata E.

Involvement of parathyroid hormone-related protein in experimental cachexia induced by a human lung cancer-derived cell line established from a bone metastasis specimen.

Int. J. Cancer 94:24-27(2001)