Normal Sugar Containing Self Prepared Serum Medium (animal Component)

Normal sugar-containing self-prepared serum medium (animal component):

Consistent with our single batch of culture media and reagents

This medium is prepared according to the blood glucose standard of normal people. When used, 5-20% fetal bovine serum should be prepared according to the experimental requirements. Mix and join CultureBoost ™ Cell growth factor can be used after treatment.

Specification: 500ml basic medium, 5mL CultureBoost ™ Cell growth factor and 10ml attachment factor ™ Cell growth coating fluid.

Normal sugar-containing self-prepared serum medium (animal component) applicable cells:

It is certified for more than 100 primary cells and cell line cultures of human and animal origin, including:

Human:

All ACBRI and CSC primary cells

Bone marrow cells (CFU-F and cell burst culture)

Connective tissue cell

dermal fibroblasts

Endothelial cells (aorta, artery, coronary artery, blood vessel and vein)

Fetal lung cells

Embryonic and adult stem cells

Fibroblast

Glomerular mesangial cells

Immortalized/tumor-derived mesenchymal cells

Microvascular cells (brain, coronary artery, dermis, glomerulus, liver, lung and retina)

smooth muscle cell

Neovascular tumor cells

Animal:

Vascular and microvascular endothelial cells of cattle and pigs

Other instructions:

     No hormone is added to the medium. The culture medium does not contain antibiotics; bacoff ® (4Z0-643) is the recommended antibiotic for all Cell Systems media. Its MIC is or higher than 2 mg/mL.

     The culture medium does not contain phenol red. Many experiments have shown that the phenol red in the cell culture medium combines with the receptors on the cell surface to make the cells react, just as the estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) at the drug level exist in the culture medium. In order to optimize the experimental conditions of researchers, unless customized, the normal Cell Systems culture medium does not contain phenol red.

And Attachment Factor ™ Better effect of using cultured cells together

In order to achieve better cell culture effect, promote cell adhesion and regeneration ability, use normal sugar-containing self-prepared serum medium (animal component) to culture cells, please use Attachment Factor according to the requirements of the instructions ™ Coated culture bottle.

Medium preservation:

Foundation: 2 - 8 ℃

5mL CultureBoost ™ Cell growth factor, 10mL Attachment Factor ™ Cell growth coating solution: - 20 ℃.

SF-4Z0-500-R culture medium is prepared by WFI. All ingredients comply with cGMP and ISO standards. The product is only used for scientific research.

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4N3-500

SDS