CultureBoost ™ Self Added Serum Cell Culture Medium (animal Component)

CultureBoost ™ Self-added serum cell culture medium (animal component):

The culture medium does not contain serum, and 5-20% serum needs to be added according to the needs of cell growth. Add the serum prepared by yourself and 5mL CultureBoost ™ The cell growth factor can be used after being added into the 500ml base.

Specification: 500ml basic medium, 5mL CultureBoost ™ Cell growth factor, 10mL Attachment Factor ™ Cell growth coating fluid.

CultureBoost ™ Self-added serum cell culture medium (animal component) Applicable cells:

It is certified for more than 100 primary cells and cell line cultures of human and animal origin, including:

Human:

All ACBRI and CSC primary cells

Bone marrow cells (CFU-F and cell burst culture)

Connective tissue cell

dermal fibroblasts

Endothelial cells (aorta, artery, coronary artery, blood vessel and vein)

Fetal lung cells

Embryonic and adult stem cells

Fibroblast

Glomerular mesangial cells

Immortalized/tumor-derived mesenchymal cells

Microvascular cells (brain, coronary artery, dermis, glomerulus, liver, lung and retina)

smooth muscle cell

Neovascular tumor cells

Animal:

Vascular and microvascular endothelial cells of cattle and pigs

Other instructions:

     No hormone is added to the medium. The culture medium does not contain antibiotics; bacoff ® (4Z0-643)is the recommended antibiotic for all Cell Systems media. Its MIC is or higher than 2 mg/mL.

     The culture medium does not contain phenol red. Many experiments have shown that the phenol red in the cell culture medium combines with the receptors on the cell surface to make the cells react, just as the estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) at the drug level exist in the culture medium. In order to optimize the experimental conditions of researchers, unless customized, the normal Cell Systems culture medium does not contain phenol red.

And Attachment Factor ™ It is better to use cultured cells together:

In order to achieve better cell culture effect and promote cell adhesion and regeneration, CultureBoost is used ™ Self-added serum cell culture medium (animal component) to culture cells, please use Attachment Factor according to the instructions ™ Coated culture bottle.

Medium preservation:

Foundation: 2 - 8 ℃

5mL CultureBoost ™ Cell growth factor, 10mL Attachment Factor ™ Cell growth coating solution: - 20 ℃.

SF-4Z0-500-R culture medium is prepared by WFI. All ingredients comply with cGMP and ISO standards. The product is only used for scientific research.

"High Circulatory Phosphate Level Is Associated with Cerebral Small-Vessel Diseases"

Chung et al. Translational Stroke Research, 2018

"α-Klotho expression determines nitric oxide synthesis in response to FGF-23 in human aortic endothelial cells" Chung et al. PLOS One, 2017

"Brain Endothelial Cells Produce Amyloid beta From Amyloid Precursor Protein 770 and Preferentially Secrete The O-Glycosylated Form" Kitazume et al Journal of Biological Chemistry, 2010.

"Mitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor alpha in Brain Endothelial CellsMitochondrial Effects of Estrogen Are Mediated by Estrogen Receptor alpha in Brain Endothelial Cells"  Razmara et al The Journal of Pharmacology and Experimental Therapeutics, 2008.

4Z3-500

SDS