RocketFuel ™ Serum Free Cell Culture Medium (animal Component)

RocketFuel ™ Serum-free cell culture medium (animal component):

       Consistent with our single batch of culture media and reagents

       The culture medium is serum-free and does not contain boletus derivative components. But RocketFuel ™ Growth factors contain bovine derived growth factors. For the supplement of non-animal-derived components, see RocketFuel-R ™。 Human serum albumin and other components from human recombinant protein are specified in the formula. Add 8ml RocketFuel ™ The cell growth factor can be used after being added into 500ml base.

Specification: 500ml basic medium, 8ml RocketFuel ™ Cell growth factor, 10ml Attachment Factor ™ Cell growth coating fluid

RocketFuel ™ Serum-free cell culture medium (animal component) Applicable cells:

It is certified for more than 100 primary cells and cell line cultures of human and animal origin, including:

Human:

All ACBRI and CSC primary cells

Bone marrow cells (CFU-F and cell burst culture)

Connective tissue cell

dermal fibroblasts

Endothelial cells (aorta, artery, coronary artery, blood vessel and vein)

Fetal lung cells

Embryonic and adult stem cells

Fibroblast

Glomerular mesangial cells

Immortalized/tumor-derived mesenchymal cells

Microvascular cells (brain, coronary artery, dermis, glomerulus, liver, lung and retina)

smooth muscle cell

Neovascular tumor cells

Animal:

Vascular and microvascular endothelial cells of cattle and pigs

Other instructions:

        No hormone is added to the medium. The culture medium does not contain antibiotics; bacoff ® (4Z0-643) is the recommended antibiotic for all Cell Systems media. Its MIC is or higher than 2 mg/mL.

        The culture medium does not contain phenol red. Many experiments have shown that the phenol red in the cell culture medium combines with the receptors on the cell surface to make the cells react, just as the estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) at the drug level exist in the culture medium. In order to optimize the experimental conditions of researchers, unless customized, the normal Cell Systems culture medium does not contain phenol red.

And Attachment Factor ™ Better effect of using cultured cells together

      In order to achieve better cell culture effect and promote cell adhesion and regeneration, RocketFuel is used ™ To culture cells in serum-free cell culture medium (animal component), please use Attachment Factor according to the instructions ™ Coated culture bottle.

Medium preservation:

Foundation: 2 - 8 ℃

8mlRocketFuel ™ Cell growth factor, 10ml Attachment Factor ™ Cell growth coating solution: - 20 ℃.

SF-4Z0-500 culture medium is prepared by WFI. All ingredients comply with cGMP and ISO standards. The product is only used for scientific research.

"Galectin‐1 studies in proliferative diabetic retinopathy" Abu El-Esrar et al, Acta Ophthalmologica, 2019.

"Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage" Xiong et al. Scientific Reports, 2018.

"Association of HMGB1 with oxidative stress markers and regulators in PDR" Abu El-Asrar and Alam et al. Molecular Vision, 2017.

"Contacting co-culture of human retinal microvascular endothelial cells alters barrier function of human embryonic stem cell derived retinal pigment epithelial cells" Skottman et al, Experimental Cell Research, 2017.

"Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice" Shen et al PLoS One, 2016.

"Optimization of an in vitro human blood–brain barrier model: Application to blood monocyte transmigration assays"  Paradis et al. MethodsX, 2015.

"The combined role of galactose-deficient IgA1and streptococcal IgA-binding M protein in inducing IL-6 and C3 secretion from human mesangial cells: implications for IgA nephropathy" Schmitt et al. J Immunology, 2014.

"Ascorbic acid prevents high glucose-induced apoptosis in human brain pericytes" May et al BioChem Biophys Research Commun. 2014.

"S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis" El-Asrar et al Molecular Vision, 2014.

"Isolation of purified H and L polypeptide chains from guinea-pig gamma-2-immunoglobulin after mild reduction" Lamm et al, Immunology, 2014.

"Gas6 stimulates angiogenesis of human retinal endothelial cells and of zebrafish embryos via ERK1/2 signaling"  Kim et al. PLoS One, 2014.

"Synthesis and biological activity of NOSH-naproxen (AVT-219) and NOSH-sulindac (AVT-18A) as potent anti-inflammatory agents with chemotherapeutic potential"  Kodela et al, Med Chem Comm. 2013.

"Alterations of retinal vasculature in cystathionine-beta-synthase mutant mice, a model of hyperhomocysteinemia" Tawflik et al, IOVS, 2013.

"Prevention of VEGF-induced growth and tube formation in human retinal endothelial cell by aldose reductase inhibition"  Yadav et al, Journal of Diabetes, 2012.

"Localization of dichlorofluorescin in cardiac myocytes: implications for assessment of oxidative stress" Swift and Sarvazyan, Am J Physiol Heart Circ Physiol, 2011.

SDS