H157 Human Oral Squamous Cell Carcinoma Cells

It was established by squamous cell carcinoma (SCC) of buccal mucosa (20 mm-40 mm) in an 84-year-old male patient. STNMP stage II, well differentiated, lymph node positive tumor. Mutant p53, codon 306, exon 8, G to A; Wild type K -, N - and Ha-ras. Subcutaneous injection and oral floor injection (in situ) did not cause tumor in nude mice, but formed epidermoid cyst subcutaneously.

Organism human

Tissue and Cell Type oral mucosa

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

89% DMEM: F12 (MD0600)+2mM glutamine (SD0007)+10% FBS (SD0200)+0.5 ug/ml hydrocortisone sodium+G/A (SD0038)

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=1692339; DOI=10.1002/path.1711600313

Prime S.S., Nixon S.V., Crane I.J., Stone A., Matthews J.B., Maitland N.J., Remnant L., Powell S.K., Game S.M., Scully C.

The behaviour of human oral squamous cell carcinoma in cell culture.

J. Pathol. 160:259-269(1990)

PubMed=1744799; DOI=10.1002/path.1711650208

Mutlu S., Matthews J.B., Midda M., Scully C., Prime S.S.

MHC antigen expression in human oral squamous carcinoma cell lines.

J. Pathol. 165:129-136(1991)

PubMed=7633286; DOI=10.1016/0964-1955(94)00030-8

Yeudall W.A., Paterson I.C., Patel V., Prime S.S.

Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.

Eur. J. Cancer B Oral Oncol. 31:136-143(1995)