BT-325 Human Glioblastoma Multiforme Cells

This cell line was established by the Beijing Institute of Neurosurgery in 1985, and has been put into storage after identification without mycoplasma/STR. This cell contains adenovirus and SV40 virus, and must be operated in the secondary biosafety platform. Please pay attention to protection. All waste liquid and utensils that have come into contact with this cell can be discarded only after high-pressure sterilization.

Animal species/Organism Human

Tissue and Cell Type glioblastoma

Morphology Fibroblast-like, adherent growth

Medium Formulation:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium ( MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

CLPUB00249

Shao W.-Z.

Establishment of a human glioblastoma multiforme cell line BT325.

Zhonghua Shen Jing Wai Ge Za Zhi 4:103-105(1988)