HCE-8693 Human Cecal Adenocarcinoma Cells (undifferentiated)

Hce-8693 is derived from the pathological section of lymph node metastasis of a male patient with undifferentiated adenocarcinoma of the cecum. Its doubling time is 30.4 hours, and its mitotic index is 28.8%. The electron microscope showed meganucleus, obvious nucleolar boundary, rich microfilaments and secretory granules. The number of chromosome patterns is 48. The rate of cloning on soft agar is 8%. CEA was positive in the supernatant of cell neutralization medium. When the xenograft was transplanted to nude mice, the tumor formed by Hce8693 cells was the same as the original lesion of the patient, and there was Alcian blue positive substance in the cytoplasm

Tissue and Cell Type undifferentiated adenocarcinoma of the cecum; Lymph node metastasis
Morphology: Epithelial like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=2634537

Zhang Z.-X., Qian L.-J., Zhang Y.-Y., Xu S.H., Qian Y.-J., Huang X.-T., Dai H.-F.

Establishment of a cell line (HCe-8693) from human undifferentiated adenocarcinoma of the cecum and its characterization.

Zhonghua Zhong Liu Za Zhi 11:413-415(1989)

PubMed=7954969

Li S., Qian L.-J., Huang X.-S.

Cytogenetic studies on a human cecum adenocarcinoma cell line (HCe-8693).

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 16:58-62(1994)