DF-1 Chicken Embryo Fibroblast

Anti-ALV-A chicken embryo fibroblast cell line; It was put into storage after identification without mycoplasma.

Animal species/Organism chicken

Tissues and Cell Type embryos, fibroblasts

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

DOI=10.1016/S2095-3119(16)61453-3

Feng M., Dai M.-M., Liao M., Cao W.-S.

Establishment of an avian leukosis virus subgroup A-resistant cell line.

J. Integr. Agric. 16:930-936(2017)

Patent=CN104560865B

Cao W.-S., Feng M., Dai M.-M., Liao M.

Cell line resistant to A subgroup avian leukosis virus as well as construction method and application thereof.

Patent number CN104560865B, 01-May-2018