THLE-3 Human immortalized hepatocytes

The THLE-3 cell line originates from primary normal hepatocytes in the left lobe of the human liver and achieves immortality through SV40 large T antigen infection. The specific process involves introducing a retroviral vector containing the Bgl I-Hpa I fragment containing SV40 T antigen into the concurrent philophilic packaging cell line PA317 to produce the virus, which then infects normal hepatocytes and establishes the THLE-3 cell line. THLE-2 and THLE-3 cell lines originated from primary hepatocytes in the same left lobe of normal liver, using the same retroviral vector (Bgl I-Hpa I fragment containing SV40 T antigen), and were produced by PA317-packaged cell lines to infect hepatocytes and thus establish the above cell lines. THLE-3 cells express phenotypic features of normal adult hepatic epithelial cells, such as cytokeratin 18 and albumin, and cytokeratin 19 is also expressed in high-pass cells during the log-growth phase. This cell line has a nearly diploid karyotype, does not express alpha-fetoprotein, and does not form tumors in nude mice without thymos. THLE-3 cells retain enzymes related to chemical tumor metabolism, including cytochrome P450 pathways, epoxide hydrolase, NADPH cytochrome P450 reductase, superoxide dismutase, catalase, glutathione S-transferases, and glutathione peroxidase can be used to absorb benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1) and other chemical carcinogens are metabolized into the final carcinogenic metabolites. These metabolites can bind to DNA, making THLE-3 cells an in vitro model for studying drug toxicology and the pathogenic and pathological studies of human hepatocellular carcinoma.

Animal Species: Human

Tissue Source:Organ: Liver Tissue: Left lobe cell type: Epithelial immortalization using SV40 macroT antigen

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:HEPM Hepatocyte Culture Medium（P2201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

DOI=10.1007/978-1-4612-0411-4_31
John F. Lechner, Duane T. Smoot, Andrea M.A. Pfeifer, Katharine H. Cole, Ainsley Weston, John Davis Groopman, Peter G. Shields, Takayoshi Tokiwa, Curtis Craig Harris;
A non-tumorigenic human liver epithelial cell culture model for chemical and biological carcinogenesis investigations.
(In book chapter) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim, Johng Sik & Dritschilo, Anatoly (eds.); pp.307-321; Humana Press; New York; USA (1990)

PubMed=7685115; DOI=10.1073/pnas.90.11.5123; PMCID=PMC46667
Andrea M.A. Pfeifer, Katherine E. Cole, Duane T. Smoot, Ainsley Weston, John Davis Groopman, Peter G. Shields, Jean-Michel Vignaud, Michel Juillerat, Michael M. Lipsky, Benjamin Franklin Trump, John F. Lechner, Curtis Craig Harris;
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.
Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Teresa A. Lehman, Ramakrishna V. Modali, Petra Boukamp, Julie Stanek, William P. Bennett, Judith A. Welsh, Robert A. Metcalf, Martha Ruskin Stampfer, Norbert E. Fusenig, Eileen M. Rogan, Curtis Craig Harris;
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

Patent=US5759765
Curtis Craig Harris, Katharine H. Cole, John F. Lechner, Roger Robert Reddel;
Human liver epithelial cell lines.
Patent number US5759765, 02-Jun-1998

PubMed=23887712; DOI=10.1038/ncomms3218; PMCID=PMC3731665
Jean-Charles Nault, Maxime Mallet, Camilla Pilati, Julien Calderaro, Paulette Bioulac-Sage, Christophe Laurent, Alexis Laurent, Daniel Cherqui, Charles Balabaud, Jessica Zucman-Rossi;
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218.1-2218.7(2013)