Bgc-sd Human gallbladder carcinoma cells -Luc

The GBC-SD cell line was established by Liu Bo, Wang Zhanmin and others in 2000 from a 61-year-old male patient with poorly differentiated gallbladder cancer. The shapes of cells are polygon, spindle and square. Secrets CEA and CA19-9. The doubling time is about 21.4 hours. It can be transplanted to nude mice. The resulting tumor is similar to the primary tumor. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

  (1) Noninvasive real-time whole-body tumor monitoring and imaging

  (2) Continuous evaluation of tumor progression and treatment response in the same animal

  (3) Transfer assessment

  (4) Reduce the need for animal sacrifice

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640(MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃

Complete cryopreservation solution: serum-free cell cryopreservation solution (SD0002)

Note: This product is for scientific research only