A549-Luc human lung cancer cells

        Human non-small cell lung cancer cell A549 is derived from white male lung cancer tissue at the age of 58, and was established by D.J. Giard et al. in 1972. M. Lieber found that A549 can synthesize high proportion of unsaturated fatty acid lecithin through the cytidine diphosphate choline pathway. The cells are not resistant to low temperature and long-term transportation after resuscitation. The minimum temperature along the route after resuscitation should be higher than 10 ℃ and the transportation route should not be too long. When A549 cells are mailed at normal temperature in winter, there will be cell shedding and necrosis. Please try to use dry ice cold chain express. This is the A549 cell line of Luc.

       Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

       In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissue source/Issue and Cell Type: lung

Morphology: epithelioid, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% F12K (MD0400)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: F12K complete medium (article number: MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only