Human fluorouracil-resistant colorectal adenocarcinoma cells HCT-15Fu

This cell line and DLD-1 (ATCCCCL-221, human colorectal adenocarcinoma) originate from the same person; However, there are still questions about isozyme and cell chromosome karyotype analysis; The cells were CSA p negative (CSA p -); If keratin is positive, the cell is a fluorouracil resistant cell line with a drug resistance index of 500 mg/ml. The drug-free culture will lead to a decrease in the drug resistance index of the cell.

Tissue and Cell Type colorectal adenocarcinoma

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)+500 mg/ml Fu

Complete medium: 1640 complete medium (article number: MD0201)+500 mg/ml Fu

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only