IOSE-80 immortalized human ovarian epidermal cells

They were taken from normal follicular cells in the ovarian tissue bank of the University of British Columbia and transfected with SV40 immortalized virus to establish the department.

Organizational source/Issue and Cell Type: Ovary

Morphology: Epithelial like, adherent growth

Medium and Culture Conditions

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Statement: The product is for scientific research only.

PubMed=1326894; DOI=10.1016/S0002-9378(11)91579-8
Maines-Bandiera S.L., Kruk P.A., Auersperg N.
Simian virus 40-transformed human ovarian surface epithelial cells escape normal growth controls but retain morphogenetic responses to extracellular matrix.
Am. J. Obstet. Gynecol. 167:729-735(1992)

PubMed=7967506
Auersperg N., Maines-Bandiera S.L., Dyck H.G., Kruk P.A.
Characterization of cultured human ovarian surface epithelial cells: phenotypic plasticity and premalignant changes.
Lab. Invest. 71:510-518(1994)

PubMed=10329039; DOI=10.1006/gyno.1999.5348
Kruk P.A., Godwin A.K., Hamilton T.C., Auersperg N.
Telomeric instability and reduced proliferative potential in ovarian surface epithelial cells from women with a family history of ovarian cancer.
Gynecol. Oncol. 73:229-236(1999)

DOI=10.5282/edoc.3898
Euer N.I.
Identification of target genes for the therapy and diagnosis of ovarian carcinoma.
Thesis PhD (2005), Ludwig Maximilians University of Munich, Germany

PubMed=15706405; DOI=10.3892/or.13.3.375

Euer N.I., Kaul S., Deissler H., Mobus V.J., Zeillinger R., Weidle U.H.
Identification of L1CAM, Jagged2 and neuromedin U as ovarian cancer-associated antigens.
Oncol. Rep. 13:375-387(2005)

PubMed=15947112; DOI=10.1677/erc.1.00896
Choi J.-H., Choi K.-C., Auersperg N., Leung P.C.K.
Gonadotropins upregulate the epidermal growth factor receptor through activation of mitogen-activated protein kinases and phosphatidyl-inositol-3-kinase in human ovarian surface epithelial cells.
Endocr. Relat. Cancer 12:407-421(2005)

PubMed=19926575; DOI=10.1309/AJCPTK87EMMIKPFS
DeRycke M.S., Andersen J.D., Harrington K.M., Pambuccian S.E., Kalloger S.E., Boylan K.L.M., Argenta P.A., Skubitz A.P.N.
S100A1 expression in ovarian and endometrial endometrioid carcinomas is a prognostic indicator of relapse-free survival.
Am. J. Clin. Pathol. 132:846-856(2009)

PubMed=23415752; DOI=10.1016/j.molonc.2012.12.007
Stordal B., Timms K., Farrelly A., Gallagher D., Busschots S., Renaud M., Thery J., Williams D., Potter J., Tran T., Korpanty G., Cremona M., Carey M.S., Li J., Li Y., Aslan O., O'Leary J.J., Mills G.B., Hennessy B.T.
BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation.
Mol. Oncol. 7:567-579(2013)