Immortalized human lung microvascular endothelial cells HULEC-5a

Transfection of human pulmonary microvascular endothelial cells with pBR-322 plasmid pSVT vector containing the large T antigen coding region of monkey virus 40A gene product HULEC-5a retains many characteristics of endothelial cells. These immortalized cells are the source of continuous regeneration of human endothelial microvascular cells and can be used as substitutes for primary human pulmonary endothelial cells for many studies.

Organizational source/Issue and Cell Type: Lung

Morphology: Epithelial like cell like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

ECGM endothelial cell culture medium (product No.: P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Statement: The product is for scientific research only

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Mehta P, et al. Entry and intracellular replication of Mycobacterium tuberculosis in cultured human microvascular endothelial cells. Microb. Pathog. 41: 119-124, 2006. PubMed: 16860530