Products Description
highQu qPCR mastermix is based on small molecule inhibitor technology, and hot start PCR allows for the highest sensitivity and specificity under standard and fast qPCR cycle conditions. They provide excellent results on both AT and GC rich templates and guarantee fast scaling, minimal or non-optimized early Ct values. ORA™ ROX L Real-time Green Fluorescent qPCR mixes offer the added advantage of simplifying the tracking process as they are coloured with an inert blue dye, making samples better visible during pipetting and processing. Our mastermix offers PCR water to ensure optimum performance. To accommodate a wide range of instruments, ORA™qPCR Green masterbatches are available in different versions - low or high ROX concentrations. For optional use, ROX passive reference dyes are premixed in ROX L and ROX H qPCR blends. If the buyer has an instrument for optional ROX testing and wishes to optionally standardize the signal, the user must select that option in the software. Note to Buyer: No U.S. patent :5,928,907 and corresponding patents outside the United States are involved in the purchase of this Product. Application: Dye-based qPCR application in low ROX concentration calibration instrument gDNA, cDNA, viral DNA, low copy number gene qPCR relative gene expression analysis, absolute quantitative product advantages: c Universal - Standard and fast cycle ideal for GC or rich templates with highest sensitivity, fast extension, early Ct value inert blue dyes, improve sample visibility and traceability Statement: Product for scientific research use only.
Publications
Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology BMC Genomics, 2017 Nov 3 Luciana Fernandes Brito, Marta Irla, Jörn Kalinowski et al. … inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum Marine Drugs, 2016 Jun 30 Nadja A. Henke, Sabine A. E. Heider, Petra Peters-Wendisch et al. amplified …using All-in HiFi polymerase (highQu, Kraichtal, Germany)...Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains Industrial Crops and Products, 2017 Nov 1 Marta Irla, Ingemar Nærdal, Trygve Brautaset et al. …The DNA amplification was carried out with ALLin™ HiFi DNA Polymerase (highQu)…Genome-Based Genetic Tool Development for Bacillus methanolicus:Theta- and Rolling Circle-Replication Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production Frontiers in Microbiology, 2016 Sep 22 Marta Irla, Tonje M. B. Heggeset, Ingemar Nærdal, Lidia Paul, Tone Haugen et al. … inserts with ALLinTM HiFi DNA Polymerase (highQu) or the ExpandTM High Fidelity PCR System (Roche)….Programming biological operating systems: Genome design, assembly and activation Nature Methods Vol 11, 2014 Daniel G Gibson …amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…Genomic, transcriptomic and phenotypic analysis of the plant growth promoting and phosphate solubilizing Paenibacillus riograndensis SBR5 Dissertation, Bielefeld University, 2017 Jun Luciana Fernandes de Brito …inserts were amplified using Allin HiFi DNA polymerase (HighQu, Kraichtal, Germany)…Role of adhesin proteins in Chlamydia infection Dissertation, Heinrich Heine University Düsseldorf, 2017 Feb Alison Favaroni …PCR with All-in HiFi Polymerase (highQu)…Charakterisierung der pH abhängigen Konformation und Multimerisierung des Influenza-A-Virus M1-Proteins Dissertation, Universität zu Lübeck, 2016 Jun Jens Klingbeil … wurde die PCR-Ansätze mit der ALLinTM HiFi DNA Polymerase (highQu) nach Herstellerangaben durchgeführt…Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum Genes, Basel, April 2018 Nadja A. Henke, Julian Wichmann, [...], and Volker F. Wendisch CR-generated fragments (All-in HiFi, highQu, Kraichtal, Germany) were assembled into isolated plasmidsVariation in ectomycorrhizal fungal communities associated with Silver linden (Tilia tomentosa) within and across urban areas FEMS Microbiology Ecology, 94, 2018 Maarten Van Geel, KangYu, Tobias Ceulemans 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and 1 μL genomic DNA.Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus Frontiers in microbiology, 2019 M Gil López, M Irla, LF Brito Inserts were amplified by polymerase chain reactions (PCRs) using ALLin TM HiFi DNA Polymerase (HighQu)Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas … Front. Bioeng. Biotechnol., 26 September 2019 M Mindt, S Hannibal… PCR-fragments were amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum Bioresource technology, 2019 M Mindt, M Heuser amplified using ALLin™ HiFi DNA Polymerase (highQu GmbH)Forest edge effects on the mycorrhizal communities of the dual-mycorrhizal tree species Alnus glutinosa (L.) Gaertn. Science of the Total Environment, 2019 M Boeraeve, O Honnay, ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, GermanySoil organic matter rather than ectomycorrhizal diversity is related to urban tree health PloS one, 2019 M Van Geel, K Yu, G Peeters 1× HiFi Buffer, 1U HiFi DNA Polymerase (HighQu, Kraichtal, Germany16. Fermentative Production of N-Alkylated Glycine Derivatives by Recombinant Corynebacterium glutamicum Using a Mutant of Imine Reductase DpkA From … 2019 - ir.niist.res.in KM Nampoothiri, M Heuser ALLin TM HiFi DNA Polymerase (highQu GmbH, Kraichtal, Germany).Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein Marine drugs, 2019 NA Henke… amplified by a high-fidelity PCR (Allin HiFi, highQu, Kraichtal, Germany)Charting the metabolic landscape of the facultative methylotroph Bacillus methanolicus bioRxiv, 2019 B Delépine, MG Lopez (PCRs) with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany)Metabolic engineering of Corynebacterium glutamicum towards methanol-dependent growth 20182019 - pub.unibielefeld.de G Hennig PCR to amplify DNA-fragments for cloning was performed with Allin HiFi polymerase (HighQu, Kraichtal, Germany) according to the manufacturer’s protocol.The impact of forest fragmentation on mycorrhizal communities Dissertation, 2019 Margaux Boeraeve DNA. ALLin HiFi Buffer and 0.25 μL ALLin HiFi DNA Polymerase (HighQu, Kraichtal, Germany).SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly Nature Structural & …, 2020 V Königs, COF Machado The proofreading thermostable ALLin RPH Polymerase (highQu) was used for the amplification of PCR fragments for cloning.Carbon Metabolism of Methylotrophic Methanogens and Asgard Archaea in Marine Sediments Dissertation, Uni Bremen, 2019 X Yin PCR was conducted with ALLin RPH polymerase Kit (highQu, Kraichtal, Germany)
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