CT26.WT Mouse Colon Adenocarcinoma Cells

CT26 is an undifferentiated colon cancer cell line induced by N-nitroso-N-methyllurethane - (NNMU). Its cloned cell line was named CT26. WT (ATCC CRL-2638). CT26. WT was stably transfected with retroviral vector LXSN containing lacZ gene encoding typical tumor associated antigen (TAA) beta-galactosidase (beta-gal) to obtain a lethal subclone CT26. CL25 (ATCC CRL-2639). Even though CT26. CL25 expressed the typical TAA beta-galactosidase, the growth rate and lethal rate of CT26. CL25 and CT26. WT in normal mice remained basically the same. Together with CT26. CL25 (ATCC CRL-2639), it can be used as a model for testing immunotherapy procedures and for studying host immune response. The culture submitted to ATCC in July 2001 contaminated mycoplasma. The offspring were treated with BM cyclin for 21 days to eliminate mycoplasma. Six weeks after treatment, mycoplasma was detected by Hoechst staining, PCR and standard culture test. The results were negative. It was put into storage after identification without mycoplasma.

Tissue source Tissue and Cell Type Organ: colon strain: BALB/c Disease: cancer

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)