C8-D1A Mouse Astrocytes

The cerebellum of C57BL/6 mice, which was born 8 days after the explant culture, was transformed spontaneously in vitro and cloned into immortalized cell lines, which were characterized by astrocytes and glial cells. It was put into storage after identification without mycoplasma.

Tissue and Cell Type cerebellum

Morphology Stellate cells, adherent growth

Medium Formulation:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium ( MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

PubMed=6466977; DOI=10.1016/0006-8993(84)90377-9

Alliot F., Pessac B.

Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella.

Brain Res. 306:283-291(1984)

PubMed=24753479; DOI=10.1002/pmic.201300495

Han D.-H., Jin J.-H., Woo J.-M., Min H., Kim Y.-S.

Proteomic analysis of mouse astrocytes and their secretome by a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.

Proteomics 14:1604-1609(2014)

PubMed=26227174; DOI=10.1002/pmic.201400623

Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)