RF/6A Monkey Choroidal Retinal (endothelial) Cells

This cell line spontaneously transformed at the early stage and has been subcultured more than 540 times. Weibel-Palade particles were observed under the electron microscope. High-generation cells are used as trophoblasts to enhance adhesion, transfer ability and supply melanocytes in the retinas. It was put into storage after identification without mycoplasma.

Tissue and Cell Type: retinal (endothelial) cells

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 basic (MD0200)+10% fetal bovine serum (SD0200)+1% GA (SD0038)

Link to complete medium: 1640 complete medium (MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=3771439; DOI=10.1007/BF02623517

Hu F., Mah K., Teramura D.J.

Gossypol effects on cultured normal and malignant melanocytes.

In Vitro Cell. Dev. Biol. 22:583-588(1986)

PubMed=3102454; DOI=10.1007/BF02623586

Lou D.-A., Hu F.

Specific antigen and organelle expression of a long-term rhesus endothelial cell line.

In Vitro Cell. Dev. Biol. 23:75-85(1987)

PubMed=27259688; DOI=10.3109/02713683.2016.1145234

Li R., Du J.-H., Chang Y.

Role of autophagy in hypoxia-induced angiogenesis of RF/6A cells in vitro.

Curr. Eye Res. 41:1566-1570(2016)