Three Antibodies (100 ×）

Sometimes appropriate concentration of antibiotics can be added to the cell culture medium to effectively prevent microbial contamination. Penicillin - streptomycin - amphotericin B (100 × Penicillin - Streptomycin-Amphotericin B Solution, 100 ×) It is a kind of three antibodies specially used for cell culture, which can be directly added to the cell culture solution after filtration. Penicillin - streptomycin - amphotericin B mixed solution (100 × The content of penicillin is 10kU/ml, the content of streptomycin is 10mg/ml, and the content of amphotericin B is 25 µ g/ml. The solution is prepared with 0.9% NaCl or PBS, and the recommended working concentration of penicillin in the cell culture medium is 100U/ml, the working concentration of streptomycin is 0.1mg/ml, and the working concentration of amphotericin B is 0.25 µ g/ml, which can be diluted by 100 times.

Amphotericin B is a kind of polyene antifungal antibiotic isolated from the culture medium of Streptomyces nodosus, which plays a role in inhibiting the growth of fungi by affecting the permeability of cell membrane.

matters needing attention:

One. Try to reduce the frequency of repeated freezing and thawing, and avoid the pollution of mother liquor during use.

Two. Pay attention to aseptic operation and try to avoid pollution.

Three. For your safety and health, please wear lab clothes and disposable gloves.

Transportation and storage:

Dry ice transportation, stored at - 20 ℃, valid for one year. It is recommended to pack and freeze separately to avoid repeated freezing and thawing.