Sp2/0-Ag14 Murine Myeloma Cells

Does not secrete immunoglobulin, for 20 μ G/ml 8-azaguanine is resistant and sensitive to HAT. The detection showed that the mouse pox virus was negative. Through mycoplasma detection in this library.

Animal species/Organism mice, NIH/Swiss

Tissue and Cell Type myeloma

Morphology lymphocyte, suspension growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is only used for scientific research.

PubMed=714156; DOI=10.1038/276269a0

Shulman M., Wilde C.D., Kohler G.

A better cell line for making hybridomas secreting specific antibodies.

Nature 276:269-270(1978)

PubMed=6769123; DOI=10.1073/pnas.77.4.2197

Kohler G.

Immunoglobulin chain loss in hybridoma lines.

Proc. Natl. Acad. Sci. U.S.A. 77:2197-2199(1980)

CLPUB00447

Mulivor R.A., Suchy S.F.

1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.

(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)