CHO Chinese Hamster Ovary Cells

It was established from the living tissue section of adult hamsters in 1957.

Animal species/Organism Chinese hamster Hamster

Gender female

Tissue and Cell Type ovary

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% F12K (MD0400)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: F12K complete medium (article number: MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=13598821; DOI=10.1084/jem.108.6.945

Puck T.T., Cieciura S.J., Robinson A.

Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.

J. Exp. Med. 108:945-956(1958)

PubMed=4120885; DOI=10.1007/BF00319690

Deaven L.L., Petersen D.F.

The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses.

Chromosoma 41:129-144(1973)

DOI=10.3390/pr1030296

Wurm F.M.

CHO quasispecies -- implications for manufacturing processes.

Processes 1:296-311(2013)

DOI=10.3390/pr5020020

Wurm F.M., Wurm M.J.

Cloning of CHO cells, productivity and genetic stability -- a discussion.

Processes 5:20.1-20.13(2017)