Human hepatic stellate cell -GFP

HLiStCsMVKupfferGFP-HLiStCsGFPHLiStCsP38

HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. GFP-HLiStCs are selected from Puromycin resistant HLiStCs after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity > 8 when cultured following the detailed protocol described below.

Medium Formulation:

Hepatocyte Culture Medium HEPM (P2201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum) (SD0001)

Declaration: This product is for scientific research only.