-GFPHuman retinal microvascular pericytes GFP-

HRMPCs2GPF-HRMPCsP4-P56

HRMPCs are initiated by elutriation from dissociated normal human retinal tissue and transfected with GFP-Lentiviral particles at passage 2. Puromycin resistant GPF-HRMPCs were selected and shipped in frozen vials (the cells are provided @ passage 4-5). Pericytes growth medium is recommended for cell culture and these cells have an average population doubling capacity > 6 when cultured following the detailed protocol described below.