Mouse liver fibroblasts-

Mouse liver fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse liver fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Medium formula: 

Fibroblast basic medium (P6000) : 500ml, Cell growth factor FGCS(P6002):5ml, Fetal bovine Serum FBS (SD0200) : 10ml, G/A(SD0038) : 5ml 

Complete medium: FGCM Fibroblast Medium（P6001）

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

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