BUG Easy To Appear In Angiogenesis Experiment

  & Nbsp; Vascular Growth Is A Key Step In Tumorigenesis. It Is Logical To Prevent Tumor Development And Metastasis By Inhibiting Tumor Angiogenesis& Nbsp; & Nbsp; Angiogenesis Is The Basis Of Reproduction, Growth And Repair. When The Tumor Has No New Blood Vessels, Its Diameter Rarely Exceeds 2 Mm-3 Mm, And Its Growth Is Dormant. When The Primary Tumor Or Metastatic Tumor Grows To A Certain Extent, The Supply Of Oxygen And Nutrients And The Excretion Of Metabolites Will Be Insufficient. Once The Blood Vessel Grows Into The Tumor, The Way Of Supplying Nutrition And Oxygen To The Tumor Tissue Will Be Changed From Peripheral Diffusion To Blood Perfusion, And Its Metabolites Can Also Be Completely Removed During Blood Circulation. Tumor Blood Vessels Not Only Provide Nutrition And Oxygen For Tumors, Transport Metabolites, But Also Determine The Pathophysiology, Growth, Invasion, Metastasis And Response To Various Treatments Of Tumors. This View Was Combed By Folkman In 1971, And Then Vascular Growth Factor Was Discovered. This Issue Has Attracted Great Attention From The Academic Community, And Related Drug Research Has Also Flourished& Nbsp; & Nbsp; & Nbsp; Tumor Angiogenesis Includes The Following Five Ways. Angiogenesis Refers To The Formation Of New Blood Vessels In Tumor Tissue By "budding" On The Basis Of The Original Microvascular Network; Vasculogenesis Refers To The Formation Of New Blood Vessels By Endothelial Progenitor Cells Derived From Blood Or Bone Marrow; Vascular Intussusception Means That Interstitial Tissue Is Incorporated Into The Existing Blood Vessels To Participate In The Formation Of Tumor Blood Vessels; Mosaic Vessels, That Is, Endothelial Cells And Scattered Tumor Cells, Are Arranged Alternately To Form Blood Vessels; Vascular Mimicry Means That Tumor Cells Simulate And Replace Endothelial Cells To Form Lumen Like Structures& Nbsp;& Nbsp; & Nbsp; In Vitro Angiogenesis Experiment Has Become A Research Hotspot. At Present, Angiogenesis Experiments Generally Use Endothelial Cell Binding Conditioned Medium Or Tumor Cells And Inoculate Them On The Substrate To Form A Three-dimensional Network Structure, Observe The Structure Formed, And Then Conduct Quantitative Analysis Through Various Software. At Present, More Efforts Have Been Made To Form Endothelial Tubules& Nbsp; & Nbsp; 1.  Endothelial Algebra Cannot Be Too Many, If HUVEC , HMEC-1 And Other Cell Lines Should Conduct Such Experiments. It Is Suggested That The Generation Of Cells Should Be Controlled Within 7 Generations. If The Cell Shape Is Bad, It Will Have A Great Relationship With Planking And Tube Formation. Therefore, When Conducting Experiments, Planking Must Be Carried Out Under The Condition That The Cells Have The Best Growth State, The Best Shape, And No Pollution. The Cells Should Be Laid As Evenly As Possible, Or The Tubules Made May Have Breakpoints& Nbsp; & Nbsp; 2.  The Primary Endothelial Cells Do The Tubule Experiment, Because The Separation Of Endothelial Cells Is Not As Simple As Peeling Off Or Endothelium As Imagined. For Example, The Cell Systems' Primary Cell Separation Standard Has A Very Specific Description. When Cells Are In P0, They Basically Have Impurities, And Then They Need To Be Screened By Magnetic Beads Or Digested By Enzymes, (In Order To Make The Experiments Of Scientific Research Users Less Affected By Antibodies, Cells In Cell Systems Generally Do Not Use Antibody Screening, So That The Primary Cells Will Not Carry Any Resistance After Purification, Which Will Affect The Final Results Of The Experiment.) Therefore, The Primary Endothelial Cells Should Try To Control The Number Of Passages When Doing The Tubule Experiment. It Is Better To Do A Passage Limit Test, And Then Revive The Cells According To The Results For Experiments& Nbsp; & Nbsp; 3.  When Laying The Board Matrix Glue, You Must Pay Attention To: It Must Be A Good Brand, A Good Brand Is Quite Expensive, So You Can't Waste It. Before Laying The Glue, You Can Dilute It With A Serum Free Medium, Usually About 1:2; (It Is Suggested That Students Who Do Not Dilute Their Hearts Should Try It. If They Dilute Too Much, They Should Try It.) A 96 Hole Plate Of Glue Is Usually About 50ul. Tuhao Can Try To Lay Glue On A 24 Hole Plate. Compared With The Down Area, It Has More Space To Take Pictures. You Can Put Up Various POSEs. Big Guys Can Try The Culture Dish Of Angiogenesis. It Is Simple And Convenient. You Can Directly Cover The Cells And Wait For The Beautiful Picture To Be Automatically Generated. Points Needing Attention: Do Not Generate Bubbles When Preparing Reagents; When Adding Cell Suspension, Do Not Let The Gun Head Touch The Matrix Glue& Nbsp; 4.  The Density Of The Cells Should Be Well Controlled. The State Is Closely Related To The Density. If You Are Careful That The Cells Do Not Form Tubes, You Can Give Them Some Growth Factors Such As EGF, VEGF, Etc& Nbsp; & Nbsp; 5.  The Time For Taking Pictures Must Be Well Controlled. Never Oversleep. Generally, Endothelial Cells Begin To Form Tubes 12 Hours After Planking, And Begin To Disintegrate 24 Hours Later;