"Follow The Rules" In Drug Screening

Birth, Old Age, Sickness And Death Are A Major Cycle Of Human Beings. Then, The Pain Brought By Illness And Pain Is Still Fresh In The Memory Of Human Beings Throughout Their Life. Therefore, Human Beings Find Ways To Treat It. If Conditions Do Not Permit, They Personally Test The Properties Of Various Drugs, Such As "Hua Tuo Tasted Hundreds Of Herbs" In Ancient China. Drug Screening Is A Step In The Modern Drug Development Process To Detect And Obtain Compounds With Specific Physiological Activity. It Is A Process Of Selecting Compounds With High Activity For A Specific Action Target From A Large Number Of Compounds Or New Compounds Through Standardized Experimental Means. In Essence, The Process Of Drug Screening Is The Process Of Conducting Pharmacological Activity Experiments On Compounds. With The Development Of Drug Development Technology, The Physiological Activity Experiments On New Compounds Have Gradually Changed From Early Validation Experiments To Screening Experiments, Which Is Called Drug Screening. As Screening, The Physiological Activities Of Different Compounds Need To Be Compared Horizontally, So The Experimental Scheme Of Drug Screening Needs To Be Standardized And Quantitative. With The Development Of Combinatorial Chemistry And Computational Chemistry, People Began To Have The Ability To Synthesize And Separate A Variety Of Compounds On A Large Scale In A Short Time. Therefore, Drug Screening Has Gradually Become One Of The Main Ways To Find Lead Compounds In The Modern New Drug Development Process. Drug Screening Models Are Generally Divided Into Biochemical Level Screening And Cell Level Screening: The Selected Cells Are Generally Primary Cells, Because The Primary Cells Can Simulate The Physiological Mechanism In The Human Body Without Any Gene Modification, So The Detection Results Are More Accurate And The Experimental Data Are More Accurate. Biochemical Drug Screening Uses The Target Of The Drug To Be Developed To Design Experiments. Generally Speaking, This Target Is A Protein With Specific Physiological Functions, Such As Enzymes And Receptors. In Addition, Some DNA With Well-defined Coding Functions Are Increasingly Becoming The Target Of Drug Action. After The Candidate Compounds Are Mixed With The Target, The Interactions Between The Compounds And The Target Can Be Quantitatively Determined By Enzyme-linked Immunosorbent Assay, Fluorescence Color Development, Nuclear Magnetic Resonance And Other Methods, Thus Becoming The Basis For Screening Compounds. Drug Screening At The Cell Level Is A Drug Screening Model That Is Closer To The Physiological Conditions. The Model Is The Target Cell Of The Drug To Be Designed. Use The Cell Culture Technology To Obtain The Required Cells, Interact These Cells With The Candidate Compounds, And Determine The Action Ability Of The Compounds Through The Detection Technology Similar To The Biochemical Level Screening, So As To Screen The Compounds. At Present, Most Of Them Follow The Following Five Rules, This Is An Optimization From The Ribinsky Five Rules. 1. The Molecular Weight Of The Compound Is Between 100 And 380 Daltons. 2. There Are As Few Hydrogen Bond Receptors As Possible In The Compound. 3. There Are As Few Aromatic Rings As Possible In The Compound. 4. The Logarithm Of The Lipid Water Partition Coefficient Of The Compound Is Between 1 And 3. 5. The Compound Can Combine With The Target Receptor. In Addition, How To Select Cells Has Become The Most Critical Factor: 1. The Cell Purity Of Screening Drugs Should Be High, Primary Cell Immunofluorescence Detection Should Not Be Less Than 2 Marker Markers; 2. The Cells Screened For Drugs Have No Resistance, And The Resistance Can Interfere With Most Of The Experimental Data, So The Primary Cells Should Not Be Screened With Antibodies During Isolation And Extraction; 3. Carry Out The Experiment When The Cell Growth Reaches Its Peak. At This Time, The Cell Metabolism Mechanism Is Complete And It Is Easier To Detect The Accuracy Of The Data. 4. Estimate The Cell Quantity. It Is Better To Use The Same Batch Of Cells In Each Group Of Experiments To Avoid The Data Difference Caused By Different Donors. Different Groups Can Use Different Donors, So As To Make A Comparison With Each Other. 5. If The Primary Cells Are Purchased, The Documents Issued By The Seniors Are Really Important. If Necessary, You Can Go Through Them In Detail To Avoid Stepping On The Pits They Have Stepped On.