What Do You Need To Do In Blood Brain Barrier Experiment?

  & Nbsp; & Nbsp;& Nbsp; At The End Of The 19th Century, Paul Ellich Discovered This Barrier In An Experiment. Paul Ellich Was A Microbiologist At That Time. He Was Studying Dyeing Technology At That Time, With The Aim Of Making Micro Biological Structures Visible. Among These Dyes, Especially Aniline Was Often Used At That Time. When Aniline Is Injected Into An Organism, All Organs Of The Organism Will Be Stained, Except For Brain Cells. At That Time, Ellich Attributed This Phenomenon To The Fact That Brain Cells Did Not Absorb Enough Dye& Nbsp; & Nbsp; & Nbsp; Many Years Later, Edwin Goldman, A Student Of Ellich, Injected Aniline Directly Into The Spinal Cord. At This Time, Brain Cells Were Stained, But Other Parts Of The Body Were Not. This Phenomenon Obviously Shows That There Is A Barrier Between The Brain And Other Body Tissues. At That Time, Because The "barrier" Could Not Be Found, The Blood Vessels Were Considered As That Barrier. This Mysterious Barrier Was Not Discovered Until The 1960s, When Scanning Electron Microscopy Was Used In Medical Research& Nbsp; & Nbsp; & Nbsp; The Cerebrovascular Barrier Hardly Allows Any Substance To Pass Through. Except For Oxygen, Carbon Dioxide And Blood Sugar, Most Drugs And Proteins Cannot Pass Through Due To Their Large Molecular Structures& Nbsp; & Nbsp; & Nbsp; & Nbsp; According To The Experimental Data, About 25% Of People Carry The APOE4 Variant Of APOE Gene, Which Greatly Increases Their Risk Of Alzheimer's Disease. Almost All Patients With Alzheimer's Disease, Even Some Elderly People Without Alzheimer's Disease, Suffer From Cerebral Amyloid Angiopathy (CAA). In This Case, Amyloid Protein Is Deposited On The Blood Vessel Wall, Which Weakens The Ability Of The Blood Brain Barrier To Correctly Transport Nutrients, Remove Wastes And Prevent Pathogens And Harmful Substances From Invading. In The Blood-brain Barrier Experiment, Good Primary Cells Have Become The Key To The Experiment, And The Extraction Of Cells Has Also Become A Key Element. One Is The Purity Of The Cells, And The Other Is My Own Operation. If It Is Not Very Good, I Will Start Again From The Beginning& Nbsp; & Nbsp; & Nbsp; The Primary Cells Of Cell Systems Represented By Shanghai Qida Biotechnology Co., Ltd. Can Solve The Problem Of Cell Sampling And Separation. When Purchasing The Primary Cells, You Must Pay Attention To Immunofluorescence Detection: Whether There Are Several Markers Of The Cells, Such As CD31, VWF/Factor VIII, Take Of Di-I-Ac-LDL Immunofluorescence Detection Of The Brain Endothelium Of Cell Systems; Human Pericytes Were Detected By Desmin Pericyte Marker, PDGFR-b Pericyte Marker, NG2 Pericyte Marker, CD13 Pericyte Marker, A-SMA Pericyte Marker And Other Major Markers Of Pericytes. Because Pericytes And Endothelial Cells Are Adjacent To Each Other, Cell Systems Also Carried Out A Series Of Endothelial Cell Marker Tests, And The Purity Was Controlled At More Than 95%. Operation Method Of Cell Culture:& Nbsp; & Nbsp; 1. The Culture Medium Is Very Important: You Must Use The Matching Culture Medium Of The Brand When You Buy Cells, Which Is Really Important, Because Cells Have Adapted To The Growth Mode When They Are Extracted. If You Change It Hastily, Cells May Play Game Over With You At Any Time. 2.  The Digestion Method Is Very Important: The Nutrient Cells Are Digested Only With Attention. If They Digest For Too Long, They Will Directly Enter The Old Age. If The Digestion Time Is Not Enough, They Will Not Be Able To Be Lifted Up Like Babies. One Lump Here And One Lump There Is A Headache, So Don't Set The Time Too Rigid When Digesting. The Digestion Time Is Closely Related To The Operation Method, Trypsin, And Digestion Environment, The Shape Of The Cell Must Prevail. When The Cell Becomes Round And The Gap Starts To Enlarge, The Digestion Can Be Terminated When The Cell On The Bottle Wall Falls Off In A Quicksand Shape By Tapping. The Digestion Package Of Cell Systems (4Z0-800) We Use Can Solve All The Digestion Problems Of Cells In One Stop& Nbsp; & Nbsp; & Nbsp; 3. Will You Choose To Freeze Primary Cells? The Local Tyrants Usually Choose To Throw Away The Cells That Cannot Be Used Up, But Is It Really Necessary? They Will Answer That The Original Generation Should Be Thrown Away, Because Few People Can Survive The Recovery. Is That True? The Method Is Very Important, Depending On How You Choose It. After 31 Years Of Experience In Cell Isolation And Cryopreservation, The Cell System Cryopreservation Solution (4Z0-705) Provides The Best Environment For Cell Freezing/thawing Cycles To Help Reduce Cell Damage. Note: It Is Only Limited To The Primary Cells That Can Be Passed On. Occasionally, When You Are Tired, You Can Freeze The Cells And Give Yourself A Holiday. Maybe The Knot That Cannot Be Opened In The Experiment Will Open. If The Experiment Is Finished, It Would Be Nice To Freeze The Cells Without Experiment And Leave Them To Younger Martial Brothers And Sisters!