Are You Right About Using Cell Culture Reagents In This Way?

 & Nbsp;& Nbsp; & Nbsp; I Have To Pay Attention To The Details Of Cell Culture Reagents. Grasp These And Control Your Cells Easily& Nbsp; & Nbsp; & Nbsp; 1. Why Should The Liquid Trypsin Solution Stored In A 4 ℃ Refrigerator Be Used Within One Week Of Dissolution? A: Trypsin May Start To Degrade At 4 ℃. If It Is Left At Room Temperature For More Than 30 Minutes, It Will Become Unstable. However, Trypsin Must Be Preheated Before Digesting Cells, Otherwise It Is Easy To Have Insufficient Enzyme Activity, Which Will Also Lead To The Inability Of Cells To Digest. Therefore, In Order To Better Culture Cells, It Can Be Used Separately When There Is Not Much Cell Culture, So That The Above Two Situations Will Not Occur, Also, When Preparing Trypsin, Pay Attention To Reducing Heat Source& Nbsp; (Left To Right Cells: HUVEC. HRMEC, HBMSC) 2. What Are The Reasons For The Slow Growth Of Cultured Cells? What Are The Solutions? Answer: The Possible Reasons Are: Different Culture Medium Or Serum Were Replaced; Some Essential Components For Cell Growth Such As Glutamine Or Growth Factors In The Culture Medium Are Depleted Or Lacking Or Have Been Destroyed; There Is A Small Amount Of Bacterial Or Fungal Contamination In The Culture; Improper Storage Of Reagents; Compare The Components Of The New Culture Medium And The Original Culture Medium, And Compare The New Serum With The Old Serum To Find Out The Possible Reasons For The Growth Of The Supporting Cells. Solution: Increase The Concentration Of Initial Cultured Cells; Let The Cells Gradually Adapt To The New Culture Medium; Replace With Freshly Prepared Culture Medium; Glutamine Or Growth Factor Supplementation; Cultivate With Antibiotic Free Culture Medium. If Contamination Is Found, Discard The Culture Or Sterilize With Antibiotics; Serum Should Be Stored At - 5 ℃ To - 20 ℃; The Culture Medium Should Be Kept Away From Light At 2-8 ℃; The Complete Culture Medium Containing Serum Shall Be Stored At 2-8 ℃ And Used Up Within 2 Weeks; Isolate The Culture And Detect Mycoplasma& Nbsp; 3. How To Eliminate The Pollution Of Tissue Culture? A: When Important Cultured Cells Are Contaminated, Researchers May Try To Eliminate Or Control The Contamination. First, Confirm That The Pollutants Are Bacteria, Fungi, Mycoplasma Or Yeast, Isolate The Polluted Cells From Other Cell Lines, Disinfect The Culture Vessels And Ultra Clean Tables With Laboratory Disinfectants, And Check The HEPA Filter& Nbsp; High Concentrations Of Antibiotics And Antimycins May Be Toxic To Some Cell Lines. Therefore, Do Dose Response Experiments To Determine The Dose Level Of Toxicity Of Antibiotics And Antimycins. This Is Particularly Important When Using Antibiotics And Antimycotics. If Mycoplasma Is Polluted, We Can Choose Our Mycoplasma Inhibitor (Qida Biological; Product No.: SD0034) To Inhibit The Growth Of Mycoplasma. It Can Clean Most Mycoplasmas, Or A Small Amount Of Stubborn Mycoplasmas. The Black Glue Insect Pollution Also Poses A Major Problem In The Laboratory At Present. The Amount And Requirements Of The Black Glue Insect Scavenger Are Very High. First, The Purification Of The Antibacterial Small Molecules Used Should Be High, Which Can Reduce The Endotoxin In The Product Itself. Second, The Amount Added. A Small Amount Will Not Clean The Black Glue Insect, But A Large Amount Will Lead To Cell Death. You Can Directly Choose Our Black Glue Insect Inhibitor (Qida Biological; Product No.: SD0036); 1ml/piece, Only 100ul Per 100ml. The Key Is The Price, Which Is Beyond Your Imagination& Nbsp;& Nbsp;& Nbsp;& Nbsp;