Benefits For Colorectal Research

The Benefits Of Colorectal Cancer Cell Research Are Coming. Here Are The Gene Expression And Culture Methods Of Colorectal Cancer Cells Sorted Out. Please Remember To Collect Them. HT-29 Human Colon Cancer Cell Article Number: CD0040HT-29 Cells Were Sampled From A 44 Year Old Woman To Express Urokinase Receptor, But Plasminogen Activator Activity Was Not Detected [PubMed ID: 8381394]. HT-29 Cells Were Negative For CD4 Expression, But The Cell Surface Expressed Galactosylceramide (an Alternative Receptor For HIV). The Oncogenes C-myc, K-ras, H-ras, N-ras, Myb, Sis, Fos Were Positive, And Excessive P53 Antigen Was Produced. There Was G - Gt At Codon 273 Of P53 Gene; A Mutation Causes Arg -> His Substitution, No Oncogene N-myc Expression Was Detected. It Is P140 When This Warehouse Is Frozen. Each Batch Passed The Detection Of Mycoplasma In This Library, And The Result Was Negative. HCT-116 Human Colorectal Cancer Cell Code: CD0039HCT116 Is One Of Three Malignant Cells Isolated From Male Patients With Colon Cancer By M. Brattain In 1979. Clones Were Formed In Semisolid Agarose Medium. HCT116 Has Tumorigenicity And Forms Epithelioid Tumor In Nude Mice Without Thymus Gland. HCT-15 Human Colorectal Adenocarcinoma Cell Product Number: CD0413DNA Fingerprinting Evidence Shows That This Cell Line And DLD-1 (ATCCCCL-221, Human Colorectal Adenocarcinoma) Originate From The Same Person; However, The Analysis Of Isozymes And Cell Karyotype Is Still Questionable; The Cells Were CSAp Negative (CSAp -); Keratin Is Positive. More Cell Characteristics Cell Lines Morphological Characteristics Culture Matrix Characteristics Caco-2 (Qida Article No.: CD0043) Colorectal Adenocarcinoma Cells Originate From Colorectal Cancer Tissues; Epithelial Cell MEM Medium (NaHCO3 1.5g/L, Sodium Pyruvate 0.11g/L) Added With 20% High-quality Fetal Bovine Serum FBS Was Isolated From Rectal Carcinoma In Situ. When Full, The Cells Showed Characteristic Differentiation Of Intestinal Epithelial Cells. Caco-2 Cells Expressed Retinoic Acid Binding Protein I And Retinoic Acid Binding Protein II, And Were Keratin Positive. COLO 205 (Qida Article Number: CD0616) People; Colon Cancer Cells; It Originates From Metastatic Ascites Of Colorectal Cancer; Epithelial, Mainly Semi Adherent Growth, A Small Part Of Adherent Growth RPMI-1640 Medium Added With 10% Fetal Bovine Serum FBS Keratin Immunoperoxidase Staining Positive. COLO320HSR (Qida Article No.: CD0619) Person; Colon Adenocarcinoma Cells Are Derived From RPMI-1640 Medium Of Colon Tissue Or DMEM Added With 10% Fetal Bovine Serum FBS. This Strain Is A Subline Of COLO 320DM (see BCRC 60108), Which Is Developed After 16 Months Of Continuous Subculture; CEA And CSAp Were Negative; Colon Antigen 3 Was Positive; The Cytokeratin And Vimentin Were Weakly Positive. In Nude Mice Inoculated With 10 (7) Cells Subcutaneously, The Tumor Occurred 80% Of The Time (4/5) Within 21 Days. COLO320DM (Qida Article No.: CD0229) Person; Colorectal Adenocarcinoma Cells Originated From Dukes' C-type Colorectal Adenocarcinoma Tissue; Round And Refractive; RPMI-1640 Medium Added With 10% Fetal Bovine Serum FBSCEA, CSAp And Colon Antigen 3 Was Negative. Keratin And Vimentin Were Weakly Positive CT26.WT (Qida Product No.: CD0203) CT26.WT-Luc Labeled Cells (product No.: CD0593) In Mice; Colon Cancer Cells Come From  Colon Cancer Tissue Of BALB/c Mice; Fibroblast RPMI-1640 Medium With 10% Fetal Bovine Serum FBSCT26; It Is An Undifferentiated Colon Cancer Cell Line Induced By NNMU. The Cell Line It Cloned Was Named CT26.WT. CT26.WT Was Stably Transfected With Retroviral Vector LXSN Containing LacZ Gene Encoding Typical Tumor Associated Antigen (TAA) Beta Galactosidase (beta Gal) To Obtain Lethal Subclone CT26.CL25 (ATCC CRL-2639)& Nbsp; Even Though CT26.CL25 Expressed Typical TAA Beta Galactosidase, The Growth Rate And Lethal Rate Of CT26.CL25 And CT26.WT In Normal Mice Were Basically Consistent& Nbsp; Together With CT26.CL25 (ATCC  CRL-2639), It Can Be Used As A Model For Testing Immunotherapy Procedures And For Studying Host Immune Response. CW-2 (article No.: CD0325) Persons; Colon Adenocarcinoma Cells Originated From Colon Tissue; Epithelial Cell RPMI-1640 Medium Added With 10% Fetal Bovine Serum FBS; CEA Positive, Transplanted Into Nude Mouse Tumorigenic DLD-1 (product No.: CD0041) Human; The Epithelial Cells Of Colorectal Adenocarcinoma Originate From The Epithelial Cells Of Colorectal Adenocarcinoma; Epithelial Cell RPMI-1640 Medium Added With 10% Fetal Bovine Serum FBS Cells Showed Negative CSA P (CSA P -). DLD-1 Cell& Nbsp; & Nbsp; The Expression Of P53 Antigen Was Positive (p53 Antigen Produced A C  -> T Point Mutation Leading To Ser -> Phe At Position 241). Keratin Immunoperoxidase Staining Was Positive. Oncogene C-myc, & Nbsp; K-ras, H-ras, N-ras,   Myb, Sis And Fos Were Positive& Nbsp; The Expression Of Oncogene N-myc Was Not Detected& Nbsp; Expression Of Tumor Specific Nuclear Matrix Proteins CC-2, CC-3, CC-4, CC-5; And  CC-6。 GPC-16 Guinea Pig; Colorectal Adenocarcinoma Originates From Colon Tissue; Epithelial Cells; Adhere To The Wall; Carcinogenic Eagle&# 39; S  MEM Medium Was Added With 10% Fetal Bovine Serum FBS Expression Gene: Keratin Gene HCT-15 (Qida Article No.: CD0413) Human; Dukes   Type C Colorectal Adenocarcinoma Cells Originate From Colorectal Adenocarcinoma Tissues; Epithelial Cells; Adhere To The Wall; Addition Of 10% Fetal Bovine Serum FBS To Carcinogenic RPMI-1640 Medium; The Cells Were CSAp Negative (CSAp -). Keratin Immunoperoxidase Staining Was Positive. Expression Of Carcinoembryonic Antigen. HCT 116 (Qida Article No.: CD0039); Colon Cancer Cells Originate From Colorectal Cancer Tissue; Epithelial Cell McCoy&# 39; The Addition Of 10% Fetal Bovine Serum FBSHCT116 To S5A Medium Has Tumorigenicity In Thymus Free Nude Mice, Forming Epithelioid Tumors. Hs 257.T