Common Sense Of Cell Culture

What Is The Difference Between In Vitro And In Vivo Culture? A: After Cells Are Isolated, They Lose The Regulation Of Neurohumors And The Interaction Between Cells. Living In A Relatively Stable Environment Lacking Dynamic Balance, They Are Prone To The Following Changes Over Time: The Differentiation Phenomenon Is Weakened; The Morphology And Function Tend To Be Unitary Or Decline And Die After Living For A Certain Time; Or It Can Be Transformed Into A Continuous Cell Line Or A Malignant Cell Line That Can Grow Indefinitely. Therefore, Cells In Culture Can Be Regarded As A Cell Population Under Specific Conditions. They Not Only Maintain The Same Basic Structure And Function As Cells In Vivo, But Also Have Some Characteristics Different From Cells In Vivo. In Fact, Once The Cells Are Placed In Vitro, This Difference Starts To Occur& Nbsp; What Are The Characteristics Of Diploid Cells? A: The Karyotype Of Diploid Cells Is 2n; Adherence Dependent Contact Inhibition; Generally, It Can Be Subcultured For 50 Generations; No Tumorigenicity. Such As W1-38: Human Diploid Cell Line Of Normal Embryonic Lung Tissue. MRC-5: Human Diploid Cell Line Obtained From Normal Male Lung Tissue. What Is Large-scale Animal Cell Culture? The So-called Large Scale Culture Technology Of Animal Cells Refers To The Technology Of Cultivating Large Quantities Of Animal Cells With High Density In A Cell Bioreactor Under Artificial Conditions (setting PH, Temperature, Dissolved Oxygen, Etc.) For The Production Of Biological Products. At Present, Animal Cells That Can Be Cultured On A Large Scale Include Chicken Embryo, Pig Kidney, Monkey Kidney, Hamster Kidney And Other Primary Cells As Well As Human Diploid Cells, CHO (Chinese Hamster Ovary) Cells, BHK-21, Vero Cells (African Green Monkey Kidney Passage Cells), And Have Successfully Produced Rabies Vaccine, Foot And Mouth Disease Vaccine, Hepatitis A Vaccine, Hepatitis B Vaccine, Erythropoietin, Monoclonal Antibody And Other Products. In The Past Few Decades, Cell Culture Technology Has Made Great Progress, From The Use Of Roller Bottle, CellCube And Other Adherent Cell Culture To The Development Of Bioreactor For Large-scale Cell Culture& Nbsp; Can The Medium Be Preserved For A Long Time After Adding Serum And Antibiotics? A: Once You Add Serum And Antibiotics To The Fresh Medium, You Should Use It Within Three To Four Weeks. Because Some Antibiotics And Basic Components In Serum Begin To Degrade After Thawing. Cell Culture Medium Is Occasionally Frozen, Can You Continue To Use It? Answer: If The Cell Culture Medium Is Accidentally Frozen, You Should Naturally Dissolve The Culture Medium And Observe Whether There Is Precipitation. If No Precipitation Occurs, The Medium Can Be Used Normally. If Precipitation Occurs, These Media Can Only Be Discarded& Nbsp; Can The Culture Medium And Other Additives And Reagents Be Frozen And Thawed Repeatedly? A: Most Of The Additives And Reagents Can Be Frozen And Thawed For Up To 3 Times. If The Number Of Times Is Too High, Will The Liquid Medium Be Kept In Cold Storage? Or Frozen? Answer: Refrigerate!! Generally, Liquid Culture Media Can Be Stored For 6 Months To One Year Under Refrigeration Conditions& Nbsp; How To Keep The Water Tray Of CO2 Incubator Clean? Answer: Replace With Sterile Distilled Water Or Sterile Deionized Water Regularly (at Least Once Every Two Weeks). Why Does The Number Of Cells In The Purchased Cell Cryotubes Become Too Small After Thawing? A: The Number Of Cells In Frozen Cell Culture Is Too Small, Mostly Because Of The Mistakes In Centrifugation Process, Resulting In Physical Damage To Cells And Cell Loss. It Is Recommended That Cells Should Not Be Centrifuged Immediately After Thawing, And Should Be Replaced Overnight After Cell Growth. What Might Be The Cause Of Cell Death Or Poor Cell Survival? A: The Survival Rate Of Researchers In Cell Culture Is Not Good. The Reasons Are Complex. Common Reasons Can Be Summarized As Follows: Wrong Use Of Culture Medium Or Poor Quality Of Culture Medium; Wrong Use Of Serum Or Poor Quality Of Serum; Unfreezing Process Error; After Thawing, The Frozen Cells Were Washed And Centrifuged; Suspension Cells Are Mistaken For Dead Cells; Wrong Use Of Culture Temperature; Cells Are Kept At – 80 ℃ For Too Long. It Is Recommended To Conduct Cell Resuscitation And Cryopreservation In Strict Accordance With ATCC Or ECACC Standard Operating Procedures. How To Cryopreservation Cells? Answer: Freezing Preservation Method 1: Place The Freezing Tube At 4 ℃ (30-60 Minutes) → - 20 ℃ (30 Minutes) → - 80 ℃ (16-18 Hours Or Overnight) → Store It In A Liquid Nitrogen Tank For A Long Time. It Is Recommended To Use The Procedure Free Cell Cryopreservation Solution From Shanghai Qida Biotechnology Co., Ltd., Without Procedure Cooling, The Low Serum And Low DMOS Series (product No.: SD0001) Do Not Need Centrifugation For Resuscitation, And Most Cells Survive In 90% - 95%, Which Is Applicable To Primary Cells, Precious Cell Culture, And The Procedure Free Serum Free Cryopreservation Solution (product No.: SD0002) Series Are Applicable To Stem Cell Cryopreservation And Tumor Cell Line Cryopreservation, The Current Activity Price Is 88 Yuan/bottle, 50ml.