MLO-Y4 小鼠骨样细胞

Derived from 14-day old mice, immortalized transfection was established; The MLO-Y4 cell line is helpful to study the effect of fluid flow and matrix stretching on the activation of specific signal pathways of bone cells. The immortalized T antigen, under the control of the osteocalcin promoter, expresses the high expression of osteocalcin, connexin 43, and the high expression of antigen E11, maintain dendritic morphology. Similar to that observed in primary bone cells, bone cells are the richest bone cells in vivo, but also the most challenging research, Because they are embedded in mineralized matrix, it is difficult to separate

Animal species/Organism mice

Tissue and Cell Type Bone

Morphology: Epithelial cell like adherent growth

Cultivation method: MEM(MD0800)+10%FBS(SD0200)+1%G/A(SD0038)

Full media link: MEM α Complete medium (MD0801)

Gas phase: 5% CO2 95% air, temperature: 37 ℃

Note: This product is only used for scientific research

PubMed=9421234; DOI=10.1359/jbmr.1997.12.12.2014
Kato Y., Windle J.J., Koop B.A., Mundy G.R., Bonewald L.F.
Establishment of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Res. 12:2014-2023(1997)

PubMed=10084404; DOI=10.1007/s007740050066
Bonewald L.F.
Establishment and characterization of an osteocyte-like cell line, MLO-Y4.
J. Bone Miner. Metab. 17:61-65(1999)

PubMed=12412815; DOI=10.1359/jbmr.2002.17.11.2068
Zhao S., Zhang Y.K., Harris S., Ahuja S.S., Bonewald L.F.
MLO-Y4 osteocyte-like cells support osteoclast formation and activation.
J. Bone Miner. Res. 17:2068-2079(2002)

PubMed=22130923; DOI=10.1007/978-1-61779-415-5_6
Rosser J., Bonewald L.F.
Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
Methods Mol. Biol. 816:67-81(2012)