Rat Retinal Cell Line (R28) Smear

Background: Each Real Cell Line (R28) Cyclopin Contains Approximately 500-1000 Cells Fixed To A Microscope Slide These Convenient Cytospins Can Immediately Be Used For Experiments Instead Of Taking The Time To Grow The Actual R28 Cells (e.g. To Quickly And Easily Check And See If The Cells Have A Particular Gene Of Interest).  The Cytospins Are Compatible With Standard Immunocytochemistry Techniques Or In Situ Hybridization.The R28 Cells Were Developed From E1A-NR.3 Parental Cell Line Through Three Rounds Of Limiting Dilution And Were Therefore Derived From A Single Cell.  Despite Their Clonal Origin, These Cells Display Both Glial And Neuronal Cell Markers Indicative Of A Retinal Precursor Cell.  The Parental Line E1A-NR.3 Was Established By Immortalization Of Postnatal Day 6 Rat Neuroretinal Tissue Using The Psi2 Replication Incompetent Retroviral Vector.  As A Result, These Cells Are Already Resistant To Geneticin/G418 And Would Require An Alternative Selection Marker For Transfection Studies.  These Cells Were Designed Not To Form Tubes In Animals. These Convenient Cell Holders Can Be Used Immediately For Experiments Rather Than Spending Time Cultivating Actual R28 Cells (for Example, Quickly And Easily Check And See If A Cell Has A Specific Gene Of Interest). These Cytoplasmic Enzymes Are Compatible With Standard Immunocytochemistry Or In Situ Hybridization Techniques. R28 Cells Are Derived From E1A-NR. 3 The Parent Cell Line Was Extracted From A Single Cell After Three Rounds Of Restricted Dilution. Although Their Origins Were Cloned, These Cells Displayed Both Glial And Neuronal Markers, Indicating Retinal Precursor Cells. Maternal Line E1A-NR. The Neuroretinal Tissue Of Postnatal Rats Was Immortalized With Psi2 Replication Unqualified Retroviral Vector. Therefore, These Cells Have Been Resistant To Genetic/G418 And Need Another Selective Marker For Transfection Research. These Cells Are Designed Not To Form Tumors In Animals. Animal Species/Oganism & Nbsp; Rat Tissue Source; Tissue And Cell Type  Morphology Of Retinal Cells; Wall Attached Growth& Nbsp; Note: This Product Is Only For Scientific Research