SK-N-MC Human Neuroepithelioma Cells

This cell line is one of two neural tissue-derived cells established by BiedlerJL (the other is SK-N-SH), which was isolated in September 1971. The cell has moderate dopamine- β- Hydroxylase activity also has intracellular catecholamines that can be indicated by formaldehyde induced fluorescence. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism Human

Tissue and Cell Type neuroepithelial tumor cells

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete freezing solution: Cell Freezing Medium (4Z0-705)

Note: This product is for scientific research only

PubMed=4748425

Biedler J.L., Helson L., Spengler B.A.

Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.

Cancer Res. 33:2643-2652(1973)

DOI=10.1007/978-1-4757-1647-4_13

Biedler J.L.

Chromosome abnormalities in human tumor cells in culture.

(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683

Biedler J.L., Spengler B.A.

A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.

J. Natl. Cancer Inst. 57:683-695(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221

Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=29704

Biedler J.L., Roffler-Tarlov S., Schachner M., Freedman L.S.

Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.

Cancer Res. 38:3751-3757(1978)

PubMed=7459858

Rousset M., Zweibaum A., Fogh J.

Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Cancer Res. 41:1165-1170(1981)

PubMed=7037175

Reynolds C.P., Reynolds D.A., Frenkel E.P., Smith R.G.

Selective toxicity of 6-hydroxydopamine and ascorbate for human neuroblastoma in vitro: a model for clearing marrow prior to autologous transplant.

Cancer Res. 42:1331-1336(1982)

PubMed=6582512; DOI=10.1073/pnas.81.2.568

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2987426; DOI=10.1007/BF00165170

Helson L., Helson C.

Human neuroblastoma cells and 13-cis-retinoic acid.

J. Neurooncol. 3:39-41(1985)

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.