MT-4 Human T-cell Leukemia Cells

The cell line was derived from the peripheral blood of a man. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism Human

Tissue and Cell Type T-cell leukemia cells

Morphology lymphoblast-like, suspension growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

1640 (MD0200)+10% FBS (SD0200)+1% G/A (SD0038)

Gas phase: air, 95%; Carbon dioxide (CO2), 5%

Freezing conditions: 55% basic culture medium+40% FBS+5% DMSO

Complete cryopreservation solution: Non programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

CLPUB00542

Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T.

Type C virus-producing cell lines derived from adult T cell leukemia.

Gann Monogr. Cancer Res. 28:219-228(1982)

PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t

Orita S., Saiga A., Takagi S., Tanaka T., Okumura K., Aono Y., Hinuma Y., Igarashi H.

A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein.

FEBS Lett. 295:127-134(1991)

PubMed=1364232

Gyuris A., Vajda G., Foldes I.

Establishment of an MT4 cell line persistently producing infective HIV-1 particles.

Acta Microbiol. Hung. 39:271-279(1992)

PubMed=8927062

Susloparov M.A., Krendel'shchikov A.V., Bakhtina M.M., Bedristov A.I., Babkin I.V., Gutorov V.V., Eroshkin A.M., Mamaeva O.A.

Cloning of proviral DNA sequences of the human T-cell leukemia virus type 1 (HTLV-1) from the genome of cultured MT-4 cells.

Mol. Genet. Mikrobiol. Virusol. 2:32-39(1996)

PubMed=9261343

Jeang K.-T., Derse D., Matocha M., Sharma O.

Expression status of Tax protein in human T-cell leukemia virus type 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH AIDS Research and Reference Reagent Program.

J. Virol. 71:6277-6278(1997)

PubMed=29892436; DOI=10.1098/rsos.172472

Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.

Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.

R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=31554688; DOI=10.1128/JVI.01390-19

Fernandez M.V., Delviks-Frankenberry K.A., Scheiblin D.A., Happel C., Pathak V.K., Freed E.O.

Authentication analysis of MT-4 cells distributed by the NIH AIDS Reagent Program.

J. Virol. 93:e01390.19-e01390.19(2019)