H-4-II-E Rat Hepatoma Cells

This cell is derived from rat liver cancer tissue and used for the detection of chloride compounds in environmental samples and food extracts. It can induce the production of aromatics hydroxylase, which is used to detect pico-grade polychlorinated biphenyl organic compounds in environmental samples or food extracts. It was put into storage after identification without mycoplasma.

Animal species/Organism rats

Tissue and Cell Type liver cancer

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

PubMed=14143233

Pitot H.C., Peraino C., Morse P.A. Jr., Potter V.R.

Hepatomas in tissue culture compared with adapting liver in vivo.

Natl. Cancer Inst. Monogr. 13:229-245(1964)