CATH. A Mouse Nerve Cells

This cell line is a cell line established from tumor culture produced in the brain of transgenic mice. The transgenic gene contains SV40T antigen coding sequence related to 783 bp partial tyrosine hydroxylase gene, and the cells express tyrosine hydroxylase and dopamine β Hydroxylase, containing neurofilament.

Animal species/Organism mice

Tissue and Cell Type nerve cells

Morphology Epithelial cell like；Adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

87% RPMI1640 (MD0200)+8% horse serum (SD0300)+4% FBS (SD0200)+2 mM/L-glutamine (SD0007)+1% G/A (SD0038)

Complete medium: RPMI1640 complete medium (MD0213)

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7680068; DOI=10.1523/JNEUROSCI.13-03-01280.1993

Suri C., Fung B.P., Tischler A.S., Chikaraishi D.M.

Catecholaminergic cell lines from the brain and adrenal glands of tyrosine hydroxylase-SV40 T antigen transgenic mice.

J. Neurosci. 13:1280-1291(1993)

Patent=US5591626

Chikaraishi D.M.

Expression of a target gene in transgenic mammals with 5' flanking sequences of the rat tyrosine hydroxylase gene.

Patent number US5591626, 07-Jan-1997

PubMed=9006967; DOI=10.1523/JNEUROSCI.17-04-01217.1997

Qi Y., Wang J.K.T., McMillian M., Chikaraishi D.M.

Characterization of a CNS cell line, CAD, in which morphological differentiation is initiated by serum deprivation.

J. Neurosci. 17:1217-1225(1997)