MA104 African Green Monkey Fetal Kidney Cell Line

MA104 cells are derived from the kidney of the monkey fetus. Cells are highly sensitive to simian rotavirus SA11. It can be used to multiply many viruses. The problematic cell line was confirmed to be the source of African green monkey.


Organizational source/Issue and Cell Type: Kidney
Morphology: Epithelial like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=4626908

Myers M.G., Vincent M.M., Hensen S.A., Tauraso N.M.

Problems in the laboratory isolation of simian hemorrhagic fever viruses and isolation of the agent responsible for the Sussex-69 epizootic.

Appl. Microbiol. 24:62-69(1972)

PubMed=4043530

Whitaker A.M., Hayward C.J.

The characterization of three monkey kidney cell lines.

Dev. Biol. Stand. 60:125-131(1985)

PubMed=16494729; DOI=10.3201/eid1201.050496

Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.

SARS-associated coronavirus replication in cell lines.

Emerg. Infect. Dis. 12:128-133(2006)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

PubMed=20143388; DOI=10.1002/ijc.25242

Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.

Check your cultures! A list of cross-contaminated or misidentified cell lines.

Int. J. Cancer 127:1-8(2010)

PubMed=26235236; DOI=10.1016/j.jviromet.2015.07.016

Otto P.H., Reetz J., Eichhorn W., Herbst W., Elschner M.C.

Isolation and propagation of the animal rotaviruses in MA-104 cells -- 30 years of practical experience.

J. Virol. Methods 223:88-95(2015)