LLC MK2 Rhesus Monkey Kidney Cells

LLC-MK2 cell line was derived from the renal cell suspension of six adult green monkeys. It is reported that this cell is sensitive to many viruses, including members of enterovirus, rhinovirus, myxovirus and poxvirus family.

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=13821019; DOI=10.1093/oxfordjournals.aje.a120078

Evans V.J., Kerr H.A., McQuilkin W.T., Earle W.R., Hull R.N.

Growth in vitro of a long-term strain of monkey-kidney cells in medium NCTC 109 free of any added protein.

Am. J. Hyg. 70:297-302(1959)

PubMed=14449901; DOI=10.1084/jem.115.5.903

Hull R.N., Cherry W.R., Tritch O.J.

Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research.

J. Exp. Med. 115:903-918(1962)

PubMed=14185313; DOI=10.1126/science.146.3641.241

Cell Culture Collection Committee

Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.

Science 146:241-243(1964)

PubMed=18605421; DOI=10.1093/jnci/45.5.951

Ikeuchi T., Sandberg A.A.

Chromosome pulverization in virus-induced heterokaryons of mammalian cells from different species.

J. Natl. Cancer Inst. 45:951-963(1970)

PubMed=4203458

Petricciani J.C., Wallace R.E., McCoy D.W.

A comparison of three in vivo assays for cell tumorigenicity.

Cancer Res. 34:105-108(1974)

PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6

McPhee D.A., Parsonson I.M., Della-Porta A.J.

Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.

Vet. Microbiol. 7:401-410(1982)

PubMed=16494729; DOI=10.3201/eid1201.050496

Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.

SARS-associated coronavirus replication in cell lines.

Emerg. Infect. Dis. 12:128-133(2006)