SPC-A-1 Human Lung Adenocarcinoma Cells

SPC-A1 was originally established in August 1980 as a lung adenocarcinoma cell line, derived from a 41-year-old male patient with primary bronchial adenocarcinoma in the right lower lobe of the lung and extensive pleural metastasis. Tissue was taken from pleural metastatic nodules and cultured in vitro to form the cell line. The cell population multiplication time was about 30 hours, and the cell morphology was dominated by pleomorphic epithelioid cells. The establishment of SPC-A1 provided an important in vitro model for studying the biological characteristics of lung cancer and its prevention and treatment. It has been reported that SPC-A1 has been contaminated with HELA. STR typing results of SPC-A-1 are consistent with HELA (partial homology).

Animal Species: Human 

Tissue Source:Lung cancer

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=22862169; DOI=10.4149/neo_2012_087
Chun-Hui Zhou, S.-F. Yang, P.-Q. Li;
Human lung cancer cell line SPC-A1 contains cells with characteristics of cancer stem cells.
Neoplasma 59:685-692(2012)

PubMed=26116706; DOI=10.1096/fj.14-266718
Fang Ye, Chu-Guang Chen, Jian Qin, Jie Liu, Cong-Yi Zheng;
Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China.
FASEB J. 29:4268-4272(2015)